Zetasizer µV - specification

The Zetasizer µV is dedicated to the measurement of the size and molecular weight of proteins, by both batch and SEC-LS modes.

Zetasizer News - changes in specification

System Specifications

General

Temperature control range

2°C - 90°C +/- 0.1°C*

Measurement angle

90°

Condensation control

Purge facility using dry air

Standard laser

60mW, 830nm

Correlator

Min. sample time 480ns, max. 3600s. 264 channels

Options

21 CFR part 11 software

Enables an operating mode that assists
with ER/ES compliance

System

Compliance

CE, Product: laser class 1, EN 60825-1:2001 and CDRH

Dimensions, weight, power

System 350mm x 410mm x 170mm (W,D,H), 14kg, 100W

Minimum PC specification

Pentium P4 PC 2.8GHz Dual core processor,
2GB RAM, 160GB hard disk drive, 1024 x 768
screen resolution, CD-ROM drive, USB port,
Windows XP Pro (SP3), Windows Vista or
Windows 7.
Note: Only the 32-bit Windows 7 and
Windows XP Pro operating systems
are supported.

Specifications for operation in cuvette mode

Size

Parameters measured

Hydrodynamic size, molecular weight
of Molecular solutions, e.g. proteins

Size range (radius)

0.15nm - 1 micron**

Minimum sample volume

2μL

Sensitivity

0.1mg/mL of 15kDa protein

Molecular weight

Molecular weight range (estimated from hydrodynamic diameter)

42Da to >10⁶Da†

Molecular weight range (calculated using Debye plot)

980Da to >10⁶Da†

Minimum sample volume

2μL

Software

Results output

Molecular size, molecular weight, A2, size distribution

Specifications for operation in chromatography detector mode

General

Parameters measured

Molecular weight, Molecular size

Flow cell volume

8μL

Molecular weight

Molecular weight range

<500Da to >10⁶Da†

Software

Results output

Mn, Mw, Mz, Mp, Mw/Mn, Rh, Weight Fraction,
Concentration, dn/dc, dA/dc. A2 ††

OmniFACE interface specification

Acquisition

2 channels RI, UV

Dimensions, weight, power

260mm x 260mm x 60mm (WxDxH)
+ power adapter, max. 65W

Note:

* Temperature accuracy, 0.1°C at 25°C, 0.2°C at 2°C and 0.5°C at 90°C

**Peak mode range (radius), 0.3nm - 0.5 microns, sample dependent

† Sample dependent

††When used in conjunction with a concentration detector