Understanding GPC/SEC

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a chromatographic technique that employs specialized columns to separate natural and synthetic polymers, biopolymers, proteins or nanoparticles on the basis of size.

As the sample is separated and elutes from the column, it can be characterized by a single concentration detector (Conventional Calibration) or series of detectors (Universal Calibration and Triple Detection).

When the GPC/SEC separation is coupled with light scattering, viscometer and concentration detectors together (triple detection), it will provide a distribution of absolute molecular weight, molecular size, and intrinsic viscosity as well as information on macromolecular structure, conformation aggregation and branching.

Separation theory:

	Separation theory How are the macromolecules separated?

Calculation types:

	Conventional Calibration For relative molecular weight measurements using a single RI or UV detector.
	Universal calibration For accurate molecular weight of polymers, branched polymers and copolymers.
	Triple Detection (Absolute Molecular Weight) Direct measurement of molecular weight, molecular size and structure for all proteins and polymers.

Detector theory:

	RI detector theory The standard concentration detector for GPC/SEC.
	Light Scattering Detector theory For absolute molecular weight measurements and triple detection of macromolecules
	Viscometer Detector theory Needed for universal calibration and triple detection.

Systems for GPC/SEC:

		Viscotek TDAmax - Integrated, temperature controlled GPC/SEC system The Viscotek TDAmax is a complete, temperature controlled, advanced, multidetector GPC/SEC system suitable for all macromolecular applications, particularly research.  It consists of three unique and complementary components – The Triple or Tetra Detector Array (TDA), the GPCmax integrated solvent and sample delivery module and the OmniSEC software.
		Viscotek 270max - Modular, ambient temperature GPC/SEC system The Viscotek 270max is a modular advanced multi detector detector system that operates at ambient temperature.  It is perfect for the routine full characterization of natural and synthetic polymers, copolymers and proteins.
		Viscotek RImax - Modular, conventional GPC/SEC system The Viscotek RImax is a modular, conventional calibration system.  It offers simple operation and full upgradeability to advanced detection.  Designed for routine GPC/SEC and teaching purposes.  Operates with the same powerful OmniSEC software as used in the advanced systems.