Zetasizer MicroV for protein characterization
Zetasizer µV

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Zetasizer µV - specification

 

The Zetasizer µV is dedicated to the measurement of the size and molecular weight of proteins, by both batch and SEC-LS modes.

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System Specifications
General Temperature control range   2°C - 90°C +/- 0.1°C*
  Measurement angle  

90°

  Condensation control   Purge facility using dry air
  Standard laser   60mW, 830nm
  Correlator   Min. sample time 480ns, max. 3600s. 264 channels
Options 21 CFR part 11 software   Enables an operating mode that assists with ER/ES compliance
System Compliance   CE, Product: laser class 1, EN 60825-1:2001 and CDRH
  Dimensions, weight, power   System 350mm x 410mm x 170mm (W,D,H), 14kg, 100W
  Minimum PC specification   Pentium P4 PC 2.8GHz Dual core processor, 2GB RAM, 160GB hard disk drive, 1024 x 768 screen resolution, CD-ROM drive, USB port, Windows XP Pro (SP3), Windows Vista or Windows 7.
Note: Only the 32-bit Windows 7 operating system is supported.
Specifications for operation in cuvette mode
  Parameters measured   Hydrodynamic size, molecular weight of Molecular solutions, e.g. proteins
Size Size range (radius)   0.15nm - 1 micron**
  Minimum sample volume   2μL
  Sensitivity   0.1mg/mL of 15kDa protein
Molecular weight Molecular weight range (estimated from
hydrodynamic diameter)
  342Da to >106Da†
  Molecular weight range (calculated using
Debye plot)
  980Da to >106Da†
  Minimum sample volume   2μL
  Software    
  Results output   Molecular size, molecular weight, A2, size distribution
Specifications for operation in chromatography detector mode
  Parameters measured   Molecular weight, Molecular size
  Flow cell volume   8μL
Molecular weight Molecular weight range   <500Da to >106Da†
  Software    
  Results output   Mn, Mw, Mz, Mp, Mw/Mn, Rh, Weight Fraction, Concentration, dn/dc, dA/dc. A2 ††
OmniFACE interface specification
  Acquisition   2 channels RI, UV
  Dimensions, weight, power   260mm x 260mm x 60mm (WxDxH) + power adapter, max. 65W

 

 

Note:

* Temperature accuracy, 0.1°C at 25°C, 0.2°C at 2°C and 0.5°C at 90°C

**Peak mode range (radius), 0.3nm - 0.5 microns, sample dependent

† Sample dependent

††When used in conjunction with a concentration detector