Size Exclusion Chromatography
Measurement of absolute molecular weight, structure, size and conformation.
Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), or Gel Filtration Chromatography (GFC) is a chromatographic technique that separates dissolved molecules on the basis of their size by pumping them through specialized columns containing a microporous packing material.
As the sample is separated and elutes from the column, it can be characterized by a single concentration detector (Conventional Calibration) or series of detectors (Universal Calibration and Triple Detection).
This technique is used to characterize natural and synthetic polymers, biopolymers, proteins or nanoparticles.
When the GPC/SEC separation is coupled with light scattering, viscometer and concentration detectors together (triple detection), it will provide a distribution of absolute molecular weight, molecular size, and intrinsic viscosity as well as information on macromolecular structure, conformation aggregation and branching.
Conventional Calibration
For relative molecular weight measurements using a single RI or UV detector.
Universal calibration
For accurate molecular weight of polymers, branched polymers and copolymers.
Direct measurement of absolute molecular weight, molecular size and structure for all proteins and polymers.
Products using Size Exclusion Chromatography
- These systems measure:
- Particle / molecular size, absolute molecular weight
- Sample type:
- Protein
- Temperature range:
- 2°C - 90°C
- These systems measure:
- Molecular weight, molecular size & molecular structure
- Sample type:
- Polymer, Protein & Biopolymer
- Temperature range:
- Ambient to 160°C
