270max Detectors and Column Oven
The Viscotek 270max is a complete, modular, advanced, multi-detector GPC/SEC system suitable for all macromolecular applications. It consists of carefully selected modular units which work seamlessly together for perfect results. The modules are detectors for light scattering, viscometry, RI, and UV, together with a column oven, the GPCmax integrated solvent and sample delivery module and the OmniSEC software.
As a Triple Detector system it employs refractive index, viscometer and light scattering detectors all acting in concert, with each detector providing complimentary data on the macromolecules being analyzed. In its Tetra Detector format, the addition of a UV detector provides a wealth of additional compositional data particularly when the optional Photodiode Array (PDA or DAD) is used to make spectral measurement of all wavelengths simultaneously.
The 270max can be configured with any combination of the 4 detectors:
- Refractive Index for concentration
- Viscometer for size and structure
- Light Scattering for molecular weight
- UV for chemical composition
In a single GPC/SEC experiment, the 270max provides:
- Absolute molecular weight without the assumptions, extrapolations or corrections required by alternative techniques.
- Molecular size expressed as Hydrodynamic Radius (Rh) to less than 1 nm and Radius of Gyration (Rg).
- Intrinsic viscosity or molecular density.
- Information on structure, conformation, aggregation and branching.
The 270max detectors and column oven:
270 Detector The Viscotek 270 Dual Detector is the ideal building block for a modular advanced GPC/SEC system. It can be specified as a dual detector (viscometer plus light scattering) or as a single detector, either viscometer or light scattering.
Refractive Index Detector The accurate 3580 Refractive Index (RI) Detector provides stable and repeatable concentration measurements for GPC/SEC measurements.
UV/VIS or Photodiode Array Detector (PDA / DAD) The Viscotek 270max offers two UV options: a programmable UV-Vis detector or the unique Viscotek PDA, the first one ever integrated in a GPC/SEC system and software.
Column Oven The VE2585 column oven is specifically designed to achieve the constant column temperature needed for repeatable chromatographic elution profiles.
Featured presentations and application notes:
Application communication: Protein and polymer molecular size by GPC/SEC The main goal of most Gel Permeation/Size Exclusion Chromatography (GPC/SEC) experiments is to determine the molecular weight distribution (MWD) of the sample or compare molecular weights (MW) of several samples.
On-demand presentation: Polysaccharide characterization by triple detection Triple detection is used with size-exclusion chromatography to characterize polymer distributions. Polysaccharides are natural polymers that can be measured using this technique. Use of triple detection allows molecular weight, size and concentration of polysaccharides to be determined and also giving information about structure. This webinar will describe the technique and provide some application examples.